FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51917112
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA558370.10755028130224192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176900.013.31734945
GGGAGGT274100.012.8336413
ATAGGGG196350.012.38720138
GGAGGTC270600.012.3826514
GGCATAG194000.012.16626335
CACCGAA237750.012.0383145
GCACCGA238950.011.99668744
ACTCTCC288200.011.43262111
GAGGTCA297550.011.3590175
GTCACTC290900.011.2960818
GAGCTTA207600.011.28294144
GGTCACT290850.011.2437417
AGGTCAC305000.010.9727126
CATAGGG232650.010.29944137
TGGCATA234700.010.1331734
TCAGACG94250.010.0662222
GCATAGG238000.010.03978236
TCACTCT342450.09.7600249
GTTGTCG31950.09.71832126
CGTGGCG137200.09.7051361
Produced by FastQC (version 0.11.5)