FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48212073
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT2743030.5689508517918324No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG920170.19085883322212677No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG750820.15573277672586283No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA680370.14112025425664648No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC553760.1148591971973493No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT506100.030.87481145
AGAACGA601100.026.02427144
AGACCGC652350.024.189024
CAGACCG663950.023.8206943
GACCGCG669650.023.5405655
AACGCTA211500.023.51702323
ACCGCGT674550.023.3782226
ACGCTAC213500.023.29672424
CGCGTTC687350.022.9592768
CCGCGTT687450.022.9461217
TCAGACC698400.022.807672
AATACGG699050.022.3977832
ATACGGA655150.022.20921733
TACGGAG659400.022.00125334
GCGTTCT719850.021.9664579
CGCTACC227300.021.88231725
TTCGACG19550.021.5180266
AAGAACG729050.021.46529643
CCCAATA735100.021.366729
CTCAGAC810750.021.2328871
Produced by FastQC (version 0.11.5)