FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48515581
Sequences flagged as poor quality0
Sequence length51-101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGA95000.037.075717
TTCGACG95400.037.035056
CGACGAG103650.033.774048
GTTCGAC104250.033.6605345
CAGTTCG121650.029.9073873
CTACGCC21500.029.21037766-67
CCTACGC22050.029.17653364-65
AGTTCGA128900.027.849514
ACCGCGT335700.026.912496
GACTAAT78950.026.77441264-65
GACCGCG339950.026.5693845
AGACCGC345000.026.2663694
CAGACCG350100.025.968443
ACCCCTA39300.025.91915370-71
CCGCGTT352850.025.5877637
CGCGTTC356050.025.3124588
GACGAGG178250.024.8279939
CCCTACG25000.024.81464464-65
CGTCTGA207950.024.56523992-93
CTAGGCG40950.024.50030582-83
Produced by FastQC (version 0.11.5)