FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48515581
Sequences flagged as poor quality0
Sequence length51-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCGA31550.041.7579562-63
GTCGAAC32200.040.91392564-65
CGTCGAA31700.038.1765364-65
TATACCG46250.036.76933350-51
CACAACG125900.033.39627582-83
CAACGTT129050.032.8176284-85
CTCGTCG38050.032.0075662-63
CGCACGC146450.029.02385182-83
GCGCGCA144750.028.67824678-79
GTAAGGT153350.028.62015770-71
AAGGTCG153150.028.25701172-73
CGAGTAT151350.027.98274492-93
GCGAGTA146000.027.906490-91
ACGTTGA122250.027.81459886-87
AACGTTG115500.027.51731784-85
GTCGGCG153050.027.32445574-75
GTAGGGA177500.027.22559294-95
CTGGCGA157700.026.70974288-89
GCACGCT154400.026.63657882-83
TCGGCGC161600.026.49399276-77
Produced by FastQC (version 0.11.5)