FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853016

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853016
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52616467
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG950560.18065827186762654No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT600380.11410496261560094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG42550.026.28046
CGGTATA67900.021.67203120
TCGACGA52450.021.6202227
CGACGAG59150.018.6767928
AGACCGC104400.018.3617574
GTTCGAC60300.018.0967065
ACCGCGT105950.018.0718986
GACCGCG107800.017.7826295
TCACGCT319200.016.7830968
AACGCTA318350.016.2628423
ACGCTAC321350.016.14601124
CAGACCG120800.016.1297073
ATGACGG88600.015.948688516
GCCGGCT320000.015.9061871
CCGGCTT366000.015.7317972
CGCTACC330600.015.64661325
CCGCGTT122700.015.6232067
TCGGCGC92000.015.50533345
TTCACGC353200.015.3713427
CCCGTAT30900.015.0742361
Produced by FastQC (version 0.11.5)