FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46192640
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT3416160.7395463866105076No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG2071270.44839827297162493No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT1018100.22040307720017732No Hit
CTCGTCTTGCTGTGTTATGCCCGCCTCTTCACGGGCAGGTCAATTTCACTG976620.21142329167590335No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG857550.1856464579638661No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC844930.18291442099866992No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA802580.17374629378186657No Hit
GTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGTCCC788180.17062891404344935No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC644170.13945295181223674No Hit
CTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGGAGCCATTCATACAGGT565700.12246539708490357No Hit
CCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCGG531780.1151222359232986No Hit
CCTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGT518180.11217804394812679No Hit
GTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTT489390.10594544931833297No Hit
GTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCT472580.10230634144313899No Hit
GGGCAGGTCAATTTCACTGGTTAAAAGTAAGAGACAGCTGAACCCTCGTGG472180.1022197475615163No Hit
CTGCATGGTTTGTCTCCCCAGTCATTGTCCACAGGACTCTGGGAAGCTGTA469680.10167853580137441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT577850.028.06750145
TTCGACG25850.024.7137036
AGACCGC694050.023.9807284
ACCGCGT698050.023.7949716
AGAACGA681500.023.79145844
CAGACCG701000.023.7839683
GACCGCG700500.023.7085385
CCGCGTT710700.023.3619177
CGCGTTC716450.023.1712828
TCAGACC734850.022.8261342
GCGTTCT749450.022.2141029
CGGTATA35100.021.78994420
GCCGGCT499700.021.7460461
TCACGCT545150.021.6508358
AAGAACG763000.021.23735443
AATACGG763500.021.23384732
ATACGGA767650.021.10732833
TACGGAG764700.021.07799134
CCGGCTT569000.020.9917762
TTCACGC574050.020.7528557
Produced by FastQC (version 0.11.5)