FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48389777
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG582240.12032293515219135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG175600.016.54861545
GGCATAG186250.015.72011135
ATAGGGG204100.014.50040838
TGGCATA211800.013.89498534
GAGCTTA214200.013.75561744
CATAGGG217700.013.43885337
GGGAGGT340200.012.9065863
GGAGGTC319600.012.7461074
GCATAGG232450.012.64418736
GTTGTCG24250.012.43251626
ACTCTCC329900.012.23365611
ACCCGGC88500.012.1492795
GTCACTC345100.011.6544328
ACGGACA91300.011.60744818
GGTCACT345300.011.510887
TAGGGGA344350.011.477303539
ATAGCTC87250.011.37597439
TCACCCG104900.011.1290413
CGGACAC101000.010.60265713
CACTCTC391800.010.39770310
Produced by FastQC (version 0.11.5)