FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853022

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853022
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48208054
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT700960.14540308969949295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC161800.021.2327164
ACCGCGT162350.021.1745766
GACCGCG163250.020.9889985
AACGCTA98750.020.36862223
ACGCTAC100700.019.93039924
CAGACCG177600.019.6097933
CGCTACC104150.019.07576825
CCGCGTT186100.018.4481097
CGCGTTC190600.018.0127038
TTCGACG20050.017.6168796
AAAACGC118750.017.05174321
TCAGACC214600.016.5957262
GAAAACG123200.016.07059120
TTCGGCG76650.016.06004744
TCGGCGC76850.015.93036645
AAACGCT134350.015.10531122
CGTACGC84550.014.7680915
GCGTTCT240400.014.3748739
CCCGTAT33350.014.3079951
CCGTACG87250.014.2852984
Produced by FastQC (version 0.11.5)