FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44532808
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG30400.023.609426
TCGACGA33900.020.9727617
CGGTATA50400.019.1066220
CGACGAG36600.018.687918
GTCCGTT37900.017.8317641
CCCGTAT28800.015.6440711
AACGACG20850.015.21539616
GTTCGAC47800.014.8269185
ATGCGCG21800.014.55227344
CTCGGTT83650.014.5425331
TCACGCT132800.014.2653448
AACGCTA123450.014.12478123
GTCCGAT27750.014.0441471
CTACACG64600.014.00119745
ACGCTAC125050.013.94405724
CCTACTC72300.013.94151218
ACCGCGT38400.013.8862626
GTTCCTA72550.013.70758215
CAGTTCG57850.013.6901283
AGACCGC39050.013.5975044
Produced by FastQC (version 0.11.5)