FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44532808
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565980.1270928166038845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG173800.016.5407445
GGCATAG184500.015.435369535
ATAGGGG194500.015.04641338
GTCACTC256950.014.2332298
GAGCTTA204650.014.10226244
GGTCACT265900.013.8133667
GGGAGGT286550.013.6968863
TGGCATA212350.013.66532934
AGGTCAC280650.013.4159776
CATAGGG217900.013.39964537
GGAGGTC281950.013.2179614
GAGGTCA293500.013.1123145
ACTCTCC283300.013.02055811
GCATAGG225500.012.87832536
TCACTCT317200.011.78515159
CACTCTC318450.011.49149610
TAGGGGA318550.011.48905639
TGGGAGG290700.010.6445092
GTTGTCG30250.010.63511226
TCTCCCC353250.010.5000613
Produced by FastQC (version 0.11.5)