FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53106275
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT609060.11468701203388866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC180700.020.601664
ACCGCGT192350.019.4233066
CAGACCG197750.018.9156763
GACCGCG199450.018.687315
CCGCGTT203100.018.3732417
CGCGTTC196100.017.6747348
TTCGACG17900.016.7075066
TTCGGCG79300.016.15468844
GAACGAT236950.015.99339945
ACGTTCG24650.015.7540476
TCAGACC247450.015.4862822
AGAACGA244750.015.38339644
ATACGGA232450.015.27564933
GCGTTCT232650.015.0610639
AATACGG246050.014.94833732
TACGGAG234300.014.81211734
TGCGCGT44500.014.34385545
CTCAGAC303000.014.3334221
ATGCGCG43950.014.32023344
CGTACGC91850.014.0933155
Produced by FastQC (version 0.11.5)