FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48217568
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG549270.11391491167700536No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA519390.10771800021104341No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG490840.10179692181903491No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG162350.015.22584345
GGCATAG170350.014.31332835
ATAGGGG179400.013.86420738
GAGCTTA186950.013.27045244
TTAGCCC74450.013.071182
GTTGTCG26100.013.0216326
TGGCATA191200.012.97597634
CATAGGG195100.012.66816137
GGGAGGT299000.012.5150193
GGAGGTC289250.012.2219174
GTCAGGA176800.011.9746041
GCATAGG211700.011.65420836
GGTCACT291800.011.6358427
GTCACTC299550.011.51452358
TAGCGTA7850.011.48033437
TGTCGTT33050.010.43835828
ACCCGGC73200.010.377385
TAGGGGA325200.010.34345939
ATATAGC39950.010.0122936
TCACCCG84150.09.8550933
Produced by FastQC (version 0.11.5)