FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853031

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853031
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48672054
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG82850.011.51235238
CATAGGG84600.011.03509537
GGCATAG85950.010.91427135
GCATAGG88300.010.59820536
AGCTTAG91950.010.54416745
ACCCGGC59050.09.4516625
TAATCGG34800.09.118354
GTAACGA36650.08.71880218
GAGCTTA118000.08.44517144
GTCACTC141550.08.1245538
TGGCATA120100.08.0356634
ATAATCG45400.07.980573
TCAGACG69000.07.8925812
GTTTCGT45950.07.835871
GTAGCGT18150.07.80819545
GGGAGGT170850.07.69239853
GGTCACT144500.07.6004237
TAGGGGA141450.07.569941539
GGTAACG40150.07.566549317
ACTCTCC166900.07.564749211
Produced by FastQC (version 0.11.5)