FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853032

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853032
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47299693
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT576590.12190142544899817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC126950.022.8279444
ACCGCGT127300.022.6711486
GACCGCG127000.022.641795
CAGACCG139850.020.931413
CCGCGTT139650.020.6497617
CGCGTTC154900.018.7038528
GAACGAT168050.017.15995245
TCAGACC180200.016.781472
TCACGCT196050.016.3613558
GCGTTCT186750.015.6584699
ATACGGA184700.015.480535533
TACGGAG184100.015.46984234
CTCAGAC230150.015.398081
ACGCTCA213550.015.21017410
AATACGG188000.015.13712732
CCGGCTT229700.014.9086782
GTCCGTT32150.014.6458351
AGAACGA202100.014.39125744
CCCGTAT34650.014.2393361
CAATACG203700.014.22465431
Produced by FastQC (version 0.11.5)