FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47299693
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG29300.014.97223726
AGCTTAG161150.013.62791945
ATAGGGG166750.013.2326538
GGCATAG165650.013.00858935
GGGAGGT276350.012.2649983
GGAGGTC252150.012.2304494
ATATAGC43300.012.1613766
GAGCTTA182100.012.09579444
GTCACTC249850.011.8730888
TGTCGTT37800.011.84223128
TGGCATA191500.011.52276734
ACTCTCC260500.011.49286411
GGTCACT259450.011.4077187
GAGGTCA280850.011.0796845
CATAGGG198450.011.01662837
AGGTCAC282750.010.9434646
GCATAGG213200.010.33900636
TATAGCT49500.010.3216087
GCTAAGT45250.010.09874314
TAGCGTA7800.010.0926937
Produced by FastQC (version 0.11.5)