FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44414856
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT33750.017.955541
GTCCGTT38950.017.5248831
CCGGCTT179300.016.6266672
GCCGGCT163150.016.5282551
ACGTTCG11900.016.4490726
TCACGCT161950.016.28238
CTACACG94450.015.65076345
CCTTACG66350.015.59861541
ACGGTAC65850.015.54632245
TTCACGC176150.014.9952797
CTCCGAT43950.014.9673161
CCCGGAT104550.014.9539321
GTCGGTT33900.014.6863051
CTCCGTT68050.014.5661421
ATGCGCG19250.014.49305944
CGGCTTC218950.014.3041953
CTCGGTT80900.014.2852821
GTCCGGT34250.014.2731271
CTCCGGT58450.014.067871
AACGCTA181400.014.05281223
Produced by FastQC (version 0.11.5)