FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853035

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853035
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44414856
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG121000.013.06928745
GGCATAG125800.012.55287735
ATAGGGG131050.012.22152538
GAGCTTA142100.011.14449944
GGGAGGT189600.011.0978323
CATAGGG143250.011.02364937
TAATCGG27300.010.716334
GCATAGG149750.010.59029736
GGAGGTC186800.010.3245064
TGGCATA154400.010.25685434
ACCCGGC63050.09.9228925
GGTCACT184950.09.8563167
GTCACTC198050.09.6134768
ACTCTCC199300.09.59832711
TAGGGGA197000.09.37473239
TCACCCG66600.09.2923023
CACCGAA154500.08.93967745
ATCGGTT39300.08.7614786
CACCCGG73300.08.688564
GACGCAA45100.08.57894444
Produced by FastQC (version 0.11.5)