FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853036

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853036
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55819589
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT111700.021.8942016
AGACCGC111900.021.8370764
GACCGCG116250.020.9424695
CCGCGTT122850.019.8699157
CGCGTTC125400.019.5017438
CAGACCG126800.019.3953153
TTCGACG23200.017.7466266
ACGCTAC120950.017.2629224
AACGCTA120450.017.22262823
TTCGGCG88950.016.87406744
CGCTACC126450.016.3698225
ATGCGCG37550.016.36035244
AATACGG119000.016.31717532
TGCGCGT37850.016.2901345
TCGGCGC91550.016.27194845
ATACGGA121550.015.95627433
GAACGAT153250.015.84385345
TCAGACC165350.015.5405492
CCCGTAT32300.015.2624781
TACGGAG125950.015.25601934
Produced by FastQC (version 0.11.5)