FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44535843
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1773010.39810855269990064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC373350.024.7366564
ACCGCGT375650.024.5193486
GACCGCG376100.024.4959095
CAGACCG386100.024.0188473
CCGCGTT394700.023.3587327
CGCGTTC403300.022.8215798
TTCGACG20900.022.713326
GAACGAT412950.021.91881245
TCAGACC431000.021.725452
GCGTTCT444650.020.7549559
CTCAGAC487750.020.2964611
GCCGGCT256250.019.4128721
AGAACGA475400.019.14356444
CGTTCTC498050.018.7690610
CCGGCTT315650.018.3248422
AATACGG497950.018.10300332
TCACGCT307650.018.0042728
TACGGAG489850.018.00285734
ATACGGA493300.017.95930133
CAATACG513250.017.6776831
Produced by FastQC (version 0.11.5)