FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853040

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853040
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47699119
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG963940.2020875899196377No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCT247350.020.8451048
GCCGGCT266850.020.3077071
AACGCTA249000.020.2336623
ACGCTAC250650.020.14535324
CCGGCTT276100.020.0045912
CGCTACC254200.019.83825
TTCACGC263750.019.6598327
ACGCTCA278400.018.48676310
AAAACGC277550.018.19279921
CGGCTTC320000.018.0406133
GAAAACG282750.017.8978620
CACGCTC296400.017.3944429
AAACGCT299200.016.981722
CGTGGTT297900.016.80292941
GTCCGTT43100.016.7087651
GTTTTCG307250.016.25434145
TCGTGGT311550.016.06581140
CTACCTC328000.015.57347127
GCTACCT331150.015.4184726
TACCTCT341650.014.95130928
Produced by FastQC (version 0.11.5)