FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47622033
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG26050.020.7288386
AACGCTA89750.018.04971523
ACGCTAC91100.017.73284524
CGCTACC93200.017.4781325
CCCGTAT32700.017.218371
GTCCGTT39200.016.489051
TCGACGA32900.016.2077927
TCACGCT112900.015.723698
CGGTATA43850.015.70088520
AAAACGC106250.015.5219921
CGACGAG34300.015.2838658
GTCGGTT48300.014.268361
ATGCGCG21300.014.26024344
ACGCTCA125600.014.11589510
CTCGGTT101100.014.0342491
GTCCGAT26200.014.0115481
GTCCCGT49700.013.9570671
CTCCGTT68650.013.9427411
TGCGCGT21900.013.86955245
CCCGGAT102050.013.6608411
Produced by FastQC (version 0.11.5)