FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47622033
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG116550.012.69944645
ATAGGGG119700.012.38408138
GGCATAG117900.012.32514835
CATAGGG123850.011.80564837
GCATAGG129500.011.36005636
TAGCGTA8400.010.71157337
GAGCTTA139850.010.68013544
GGGAGGT198400.010.4468693
TGGCATA147850.010.05671834
GGAGGTC189550.09.9374484
TAGGGGA181950.09.74193639
GGTCACT185250.09.7314517
GTCACTC186450.09.7292038
ACTCTCC195400.09.61765711
ACCCGGC66950.09.1431165
CGTACGC13500.09.0019365
GTTGTCG22450.08.91906126
AGGTCAC217750.08.9094446
GAGGTCA225850.08.6392285
TAATCGG32600.08.4220044
Produced by FastQC (version 0.11.5)