FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853044

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853044
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51956407
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT742160.14284282590980552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC188900.024.6039354
GACCGCG190350.024.4518265
ACCGCGT190600.024.4075536
CAGACCG198300.023.5626773
CCGCGTT203500.022.882457
CGCGTTC215200.021.6906458
GAACGAT217250.021.36517545
TCAGACC244700.019.6007392
AATACGG240600.019.29262732
ATACGGA246050.018.83960233
AGAACGA250100.018.66691844
GCGTTCT254000.018.4924059
CAATACG259750.017.93985731
CTCAGAC313600.017.5733761
AAGAACG270000.017.24932943
TACGGAG277850.016.65906134
CCCAATA289300.016.27820229
AACGCTA172950.015.92366923
ACGCTAC173250.015.77892524
CGCTACC178100.015.32426425
Produced by FastQC (version 0.11.5)