FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51956407
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG810620.15601925668185637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCACT375450.016.5935337
GGGAGGT400400.016.4584483
GTCACTC386300.016.2270588
AGCTTAG252400.016.11731745
AGGTCAC399900.015.8712876
GGAGGTC413550.015.4501634
GGCATAG270500.015.23243235
ATAGGGG271250.015.20473738
ACTCTCC412950.015.15892411
GAGGTCA429700.014.9956995
TGGCATA295450.014.015264534
GAGCTTA294700.013.92606444
TCACTCT462850.013.7913239
CACTCTC473550.013.28972810
TGGGAGG333200.013.094722
CATAGGG325500.012.6710937
TAGGGGA494100.012.57726539
TCTCCCC503200.012.5445213
GTTGTCG26600.012.43754226
AGCATAG173500.012.281013545
Produced by FastQC (version 0.11.5)