FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48200526
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT665450.13805865935985845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC131900.024.5408424
GACCGCG132450.024.455935
ACCGCGT132800.024.3596846
CAGACCG140600.023.3429723
CCGCGTT142950.022.661537
GAACGAT152650.021.88540645
CGCGTTC156300.021.603928
AACGCTA77500.020.89977823
ACGCTAC78600.020.72209224
TTCGACG18900.020.2347566
AATACGG165750.020.15650732
ATACGGA170250.019.79552733
CGCTACC85050.019.15057625
AGAACGA174650.019.12830244
TCAGACC179700.018.6398892
TACGGAG181950.018.41134534
CAATACG188600.017.78576331
AAGAACG188800.017.73043643
TTCGGCG81750.017.28185744
AAAACGC95550.017.2106621
Produced by FastQC (version 0.11.5)