FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48200526
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG160250.015.364945
GGCATAG160700.015.20358135
ATAGGGG167350.014.82650938
GTCACTC244950.014.2752118
CATAGGG176950.013.98428437
GAGCTTA179500.013.81034344
GGTCACT251650.013.7165857
AGGTCAC269150.013.2256516
TGGCATA188550.013.17345634
ACTCTCC271600.013.09769611
GGGAGGT291450.013.0214793
GCATAGG195650.012.70567336
GAGGTCA290700.012.4816485
GGAGGTC304550.012.025274
TAGCGTA10350.011.7368537
TAGGGGA300250.011.30544639
TCACTCT327100.010.9928939
CGTCGAT24300.010.64866412
GCGTAGG91750.010.61688336
CGTAGGG93150.010.43275537
Produced by FastQC (version 0.11.5)