FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853054

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853054
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49025964
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1018360.20771850605528125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC199900.025.4256884
ACCGCGT203300.024.9672686
GAACGAT202400.024.66698345
GACCGCG207650.024.4225645
CAGACCG209700.024.3447513
CCGCGTT214050.023.702857
CGCGTTC216750.023.397218
AGAACGA236500.021.15791744
TCAGACC250900.020.7237592
AATACGG244150.020.56869532
ATACGGA244900.020.56082533
TACGGAG246750.020.26989734
GCGTTCT261400.019.5384259
CTCAGAC330350.018.7821671
TTCGACG21800.018.6806226
ACGGAGA276850.018.17986935
TTCGGCG121050.017.93625544
TCGGCGC121650.017.75531645
CCCAATA288100.017.71992329
CGGAGAG293000.017.07797636
Produced by FastQC (version 0.11.5)