FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853055

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853055
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49025964
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG132250.016.9622143
ACCCGGC130700.016.6797355
ACGGACA137650.015.56105118
CGGACAC153400.014.43206313
CCGGACA157350.014.1413512
CGGACAG151050.013.97205519
CACCCGG164500.013.6772564
GACACGG159400.013.39543915
TAGCGTA18150.013.26691637
CACGGAC164850.013.0071917
GTTGTCG24050.013.00332526
ACACGGA165250.012.96208916
ATAGCTC165900.012.49329439
GCCCGGA177800.012.46420210
TCAGACG93250.012.4272152
GGACACG182250.011.715692514
ATAGCGT20550.011.607285536
CGGCCCG193450.011.4787528
TAGCTCT181350.011.46602240
CGTCGAT35200.011.31336912
Produced by FastQC (version 0.11.5)