FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853056

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853056
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49891647
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG527050.1056389258907408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG33450.025.0873766
TCGACGA38100.022.497937
CGGTATA57100.020.05591820
AGACCGC133950.018.592974
CGACGAG45800.018.1754198
ACCGCGT137950.018.07016
GACCGCG137950.018.0538465
CAGACCG145400.017.4537413
CCGCGTT152400.016.3715257
GTTCGAC52050.016.3386025
CGCGTTC155650.016.0588028
GAACGAT157500.015.87453545
TCACGCT195700.015.4854918
GTCCGTT35250.014.7500641
GCCGGCT198850.014.7036311
CCGTACG60450.014.5148364
CGTACGC61250.014.3252555
CCGGCTT225500.014.2470682
ACGCTAC203450.014.15581524
TTCACGC218400.014.1436167
Produced by FastQC (version 0.11.5)