FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853057

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853057
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49891647
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG139600.014.42998645
GTTGTCG35350.014.00045426
ATAGGGG149000.013.86491538
GGCATAG142500.013.83301835
CATAGGG161100.012.43187237
GAGCTTA165850.012.21397744
GCATAGG166850.011.97617136
TGTCGTT41950.011.90501228
TGGCATA171750.011.60815934
GGGAGGT250650.011.391063
GTCACTC229050.011.3052128
GGTCACT233350.011.1163937
GGAGGTC252450.010.8196584
CACCGAA248500.010.76920845
TATAGCT58250.010.6599597
ACTCTCC254150.010.3837711
GCACCGA234250.010.27136444
GCTAAGT52150.010.181807514
ATATAGC60650.09.9784486
TCGTTCC53450.09.63813830
Produced by FastQC (version 0.11.5)