FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853058

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853058
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51085490
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG1208140.23649376760406918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG60250.031.032696
TCGACGA67750.028.0954827
GTTCGAC70000.026.8071275
CGGTATA96850.026.5999820
CGACGAG70700.026.1912278
AGTTCGA87050.022.1252214
ACGAGGA114150.020.57785210
CGAGGAT107250.020.26540411
GCCGGCT330450.020.2287851
GTATAGA137600.020.11231822
CAGTTCG99150.020.0605223
GACGAGG116500.019.9116949
ATGACGG121950.019.88897516
CCGGCTT384900.018.9927332
TCACGCT384400.018.7126248
TATAGAG153100.018.70805723
ATAGAGT150250.018.5687524
TTCACGC395050.018.293597
GACGGTA142900.018.10677318
ACGCTCA415050.017.40665610
Produced by FastQC (version 0.11.5)