FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853060

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853060
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51354521
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG614890.11973434627109071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTAC185550.019.05058524
AACGCTA186700.018.9572523
GCCGGCT196500.018.8064381
CGCTACC190350.018.45234525
CCGGCTT218900.018.3890342
TCACGCT212350.017.714078
GTCCGTT51500.017.6436311
AAAACGC207500.017.08817521
TTCACGC231750.016.551567
CCCGTAT36900.016.4978561
GAAAACG219600.016.13636820
AAACGCT223050.016.01798622
ACGCTCA236000.015.97697610
CCCGGAT136000.015.517691
CGGCTTC276600.015.3909353
CGTGGTT231400.015.15720741
GTTTTCG241600.014.87198545
TCGTGGT235400.014.87097840
GTCCGGT35950.014.1742361
CACGCTC267200.014.0609399
Produced by FastQC (version 0.11.5)