FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55484498
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG577840.10414440444248049No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCCG29700.020.45205732
ACGTTCG28250.018.873816
ACCGCGT112950.018.6431486
GACCGCG117200.017.954185
AGACCGC117450.017.8968984
AACGCTA233950.017.64583823
ACGCTAC234200.017.63660824
TTCGACG23050.017.5683616
GCCGGCT251500.017.4700471
CCGGCTT284750.016.8903242
TCACGCT205450.016.841458
CGCTACC248600.016.5969225
CCGCGTT127200.016.4838437
CAGACCG130250.016.466923
CGCGTTC128300.016.2898968
AAAACGC256900.016.23584621
TCGTATG40350.015.25920345
TTCACGC236000.015.1093897
CACGCTC230000.015.0633719
GAAAACG277750.015.05756120
Produced by FastQC (version 0.11.5)