FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55484498
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG633520.11417963986986059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC119100.014.2801325
GTTGTCG38100.013.93894126
GCGTCGT43100.013.72766711
CCGGACA122350.013.69842412
AGCTTAG255250.013.32630645
TCGTGTA44800.013.30735114
CGGACAC122000.013.23994113
GGCATAG258150.013.13662835
GGAGGTC349450.013.0241774
GGGAGGT377800.012.9581273
TCACCCG133200.012.938083
GGTCGCC45050.012.78381143
ACGGACA126400.012.72652218
GGTCACT359250.012.5368697
ATAGGGG279150.012.36495538
CACCCGG144150.012.282864
GTCACTC368500.012.2648318
GAGGTCA384750.012.1386645
TGGCATA288250.011.94465234
GAGCTTA286500.011.94317444
Produced by FastQC (version 0.11.5)