FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853066

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853066
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50605338
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA716070.14150088277248538No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG683200.13500552056385828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG65850.030.8401176
GTTCGAC68250.029.6178195
CGACGAG72050.027.7180718
TCGACGA78500.025.813047
CGGTATA114650.024.01000620
ATGACGG113050.022.6197916
AGTTCGA91600.022.6100444
GACGAGG113250.021.9436119
ACGAGGA116550.021.61174210
GCCGGCT197800.020.6534711
CCGGCTT222600.020.3026082
CAGTTCG112650.019.545853
GACCGCG73650.019.3684225
TGACGGT135700.018.8608417
ACCGCGT77600.018.693136
TATAGAG159500.018.68268423
GACGGTA148200.018.57512918
TCACGCT242600.018.4107918
ATAGAGT162050.018.36093724
TTCACGC246650.018.2726127
Produced by FastQC (version 0.11.5)