FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853068

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853068
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48999694
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG641100.1308375517610375No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC149550.024.1623954
AACGCTA142550.024.00715323
ACGCTAC146450.023.39856524
CAGACCG155900.023.3369883
GACCGCG155750.023.2005565
ACCGCGT159450.022.6480836
CGCTACC154200.022.164225
AAAACGC162100.021.22282221
GAAAACG175700.019.59288420
CCGCGTT184800.019.5413047
AAACGCT184500.018.75593222
TTCGACG19500.018.692246
TCAGACC201950.018.3498082
CGCGTTC199750.018.1124828
TCACGCT203150.017.6432118
GTTTTCG203000.016.86951445
TTCGGCG119650.016.49190744
TCGTGGT210800.016.38400340
CGTGGTT214800.016.0473641
ACGCTCA232600.015.85433110
Produced by FastQC (version 0.11.5)