FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48999694
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG554970.11325989096993136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGC109850.016.4933665
GTTGTCG30400.016.42909826
AGCTTAG160550.016.19868345
GGCATAG173750.015.00706235
ATAGCTC113400.014.7606339
TCACCCG126400.014.690463
GTCACTC253000.014.3014228
ACGGACA127100.014.28509718
GAGCTTA185250.014.23316844
CGGACAC130400.014.02899813
ATAGGGG189900.013.92025738
GGTCACT266700.013.5673747
TGTCGTT36950.013.51687928
CATAGGG194650.013.47655737
ACACGGA134800.013.35222116
TAGCTCT126900.013.24353740
CGGACAG133550.013.24187119
ACTCTCC279150.013.06653611
TGGCATA203150.012.94556434
AGGTCAC289500.012.9358566
Produced by FastQC (version 0.11.5)