FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44773338
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1581400.35320127349004No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG1566400.3498510653818127No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG975010.21776576050684449No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAG808520.18058068397759397No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG793070.1771299696261199No Hit
CACCCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCA720000.16080998919490883No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG713410.15933813109936096No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGA673840.15050028210985744No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC667640.1491155294251235No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGT644570.14396290935466996No Hit
CTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGC585280.13072065343888364No Hit
CCGAAAACCACGAGGAAGAGAGGTAGCGTTTTCTCCTGAGCGTGAAGCCGG557830.12458977260082775No Hit
TTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGT470300.10504019155328558No Hit
TCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA462630.10332711847394536No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA452660.1011003468180103No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGGG182500.020.1295762
CACCCGG211050.018.0131721
TGTCGTT37100.017.1591828
GTTGTCG38900.016.54827926
ATATAGC42150.016.24926
AGGGATC305100.016.0372262
GGGAGGT581700.015.7460013
AGGTCAC568600.015.7459596
GGTCACT561550.015.7323517
GTCACTC561050.015.6946348
GAGGTCA581500.015.6318145
TATAGCT44950.015.4374837
GGAGGTC600350.015.129834
TGGGAGG358750.014.8406832
CAGGGAT348250.014.7337621
TGCCCGT272750.014.54726344
ACTCTCC609950.014.38939411
TCACTCT632750.014.026279
CCCGGGA266650.013.8949843
GCTAAGT47150.013.67048114
Produced by FastQC (version 0.11.5)