FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42970128
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCGG31500.012.9294264
GGTAACG31800.011.81800217
AATCGGT37700.011.4596845
GCACCGA131850.011.19260944
CACCGAA136300.010.92621545
GTAACGA36250.010.30464618
ATAATCG43800.09.9150163
GCGTCGT26400.09.6326311
ATCGGTT43650.09.4336366
GTCGCCG26600.09.38747844
CGATCGA14300.08.809792540
ATAGGGG73450.08.69856938
CGTAACG12600.08.56998142
GGTCGCC28050.08.42101843
CGTGTAG175800.08.2667936
TTAACGC27400.08.12841632
CCTAGAT72350.08.0857021
CATAGGG79500.07.923410437
AGCACCG189550.07.88047543
ACGATCG16350.07.8429439
Produced by FastQC (version 0.11.5)