FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46827963
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1250660.26707546514461883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT258100.025.3768445
ACCGCGT271550.024.535336
GACCGCG271950.024.4992395
AGACCGC277500.024.0660154
CCGCGTT280300.023.7435637
TTCGACG31000.023.662426
CGCGTTC283350.023.4800458
CAGACCG287700.023.2831763
TCAGACC320500.021.1882232
CGGTATA47400.021.07538220
AGAACGA312300.021.0447144
GCGTTCT327700.020.357259
TCGACGA35900.020.3058597
AATACGG324450.020.16589232
ATACGGA330900.019.77281233
TACGGAG331650.019.59241934
CTCAGAC386650.019.1336461
CAATACG327300.018.94541231
CGACGAG37950.018.793968
AAGAACG352900.018.61085143
Produced by FastQC (version 0.11.5)