FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853080

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853080
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50899447
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT523910.10293039136554862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG118400.020.941055
AGACCGC119450.020.8323144
ACCGCGT119850.020.6876956
CAGACCG131950.019.0804773
CCGCGTT134900.018.4297267
TTCGACG18700.017.9272296
CGCGTTC144750.017.1911568
AACGCTA154900.017.08143823
ACGCTAC156000.017.0042624
GAACGAT147600.016.99640345
CGCTACC160800.016.41271825
GTCCGTT35150.016.2170261
TCACGCT179800.015.7044438
AATACGG161700.015.52836432
ATACGGA162350.015.5077733
TTCGGCG69300.015.38911444
AAAACGC175050.015.33369421
TTCACGC190750.014.8972927
TCAGACC177050.014.8809092
TACGGAG169550.014.80941534
Produced by FastQC (version 0.11.5)