FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853081

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853081
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50899447
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG127850.014.32631638
GGCATAG130300.013.96997935
AGCTTAG133500.013.58487545
GGGAGGT295500.013.3350883
GTCACTC289200.012.9297798
GGAGGTC306450.012.4616434
GGTCACT315000.011.8709677
ACTCTCC317100.011.82768811
GAGCTTA158200.011.8196644
TGGCATA163650.011.34304334
AGCATAG165950.011.24033345
CACCCGG118000.011.0259691
AGGTCAC360050.010.9233046
GAGGTCA366350.010.8463715
GCGTAGG176200.010.50985336
TAGGGGA353600.010.442533539
CACTCTC359650.010.43461710
GGCGTAG177950.010.40623235
TCACTCT368200.010.4000199
CGTAGGG177750.010.39299137
Produced by FastQC (version 0.11.5)