FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853082

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853082
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40287809
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG456730.11336680036385201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG24600.022.1306926
TCGACGA28150.018.8602897
CGACGAG29600.018.3923998
AACGCTA131450.018.12810923
ACGCTAC132850.017.97235324
GTCCGTT31400.017.5759831
CGCTACC137550.017.30940425
CGGTATA41550.016.62352220
CCGGCTT179100.016.216062
TTCACGC154350.016.2073827
AAAACGC149600.016.10726221
ACGCTCA165300.015.88227610
CCCGTAT24850.015.6820531
GCCGGCT163600.015.5038041
GAAAACG159100.015.13105920
AAACGCT163400.014.81688422
CGGCTTC207400.014.6541733
CACGCTC176500.014.2626479
GTTCGAC38100.013.8757875
ACCGCGT31950.013.730256
Produced by FastQC (version 0.11.5)