FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853084

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853084
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52531371
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT638930.12162827427443308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG124650.024.72845
ACCGCGT125350.024.6441546
CCGCGTT132300.023.2815197
AGACCGC136200.022.6644254
CGCGTTC136550.022.5733788
CAGACCG144450.021.5880493
GAACGAT148900.020.26290945
TTCGACG24800.019.0517716
TACGGAG170700.017.5734
ATACGGA172850.017.4165433
AATACGG175050.017.27477332
TCAGACC186500.017.0825272
GCGTTCT185750.016.7396729
AGAACGA190150.016.12750644
CAATACG190300.016.11507231
CGGTATA37800.016.07088320
AAGAACG201250.015.2491743
CGACGAG30250.015.2474148
TCGACGA31500.015.0709157
ACGGAGA202200.015.06650935
Produced by FastQC (version 0.11.5)