FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50468810
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT99100.020.9553436
GACCGCG104750.019.8035765
AGACCGC109600.019.1119884
CCGCGTT109550.018.8947967
CAGACCG117200.018.2373923
CGCGTTC116550.017.8564958
TTCGACG17200.017.2668156
TCGGCGC63150.016.28206845
AACGCTA164650.016.02887323
ACGCTAC164550.015.9702524
TTCGGCG65350.015.73393544
TCACGCT183900.015.7335248
CGCTACC168000.015.58871925
CCCGTAT31650.015.3077831
CCGGCTT207350.015.0283922
GAACGAT144500.014.66728745
TTCACGC202950.014.5670997
ACGCTCA203800.014.46218310
GCCGGCT198900.014.4111911
AAAACGC189150.014.17870821
Produced by FastQC (version 0.11.5)