FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853090

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853090
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45734994
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1242400.2716519433674792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37500.026.3350546
GACCGCG232250.026.0714875
ACCGCGT235200.025.7859786
AGACCGC237100.025.5762274
GAACGAT239050.025.32263445
CAGACCG246250.024.7812233
CCGCGTT248550.024.4001987
CGCGTTC251300.024.1242318
TCGACGA44150.022.4186387
CGGTATA59200.022.0387720
AGAACGA278150.021.78715544
TCAGACC284200.021.7808762
CGACGAG45150.021.7228098
TACGGAG278050.021.66234834
AATACGG278850.021.60871932
ATACGGA281000.021.49144733
GCGTTCT296050.020.5533819
CAATACG295800.020.40092831
AAGAACG302550.020.00032843
ACGGAGA309750.019.50266835
Produced by FastQC (version 0.11.5)