FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63535883
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1353550.2130370959037431No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT263500.025.82213245
AGACCGC291650.023.5876064
ACCGCGT292600.023.4707976
CCGCGTT305100.022.4870787
CAGACCG309400.022.2999083
CGCGTTC312200.022.0045018
AGAACGA313100.021.85034444
GACCGCG315550.021.7939265
ATACGGA342950.019.85299333
AATACGG344450.019.81923132
TCAGACC355000.019.7853572
TACGGAG345450.019.73501434
GCGTTCT364850.018.9462649
CAATACG378750.018.07122431
AAGAACG381250.017.9409243
ACGGAGA388150.017.65611835
TCGGCGC98100.017.27097545
TTCGGCG101550.017.12476244
CCCAATA402650.017.10485329
CTCAGAC467150.016.8396151
Produced by FastQC (version 0.11.5)