FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853094

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853094
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51502422
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG38850.023.5941566
TCGACGA46800.020.3542587
AACGCTA127800.019.70652623
CGGTATA59200.019.54442620
ACGCTAC128850.019.42395824
CGACGAG49850.018.3427548
CGCTACC139300.017.70876125
AAAACGC144850.017.40245421
TCACGCT164150.016.1912048
GAAAACG161900.015.87510520
AAACGCT165150.015.4537922
GTTCGAC60900.015.0883925
GACCGCG56700.014.5022375
ACCGCGT57250.014.4413436
ACGCTCA192200.014.2607310
TTCACGC195200.014.0415577
CAGTTCG72850.013.8778183
CCCGTAT35650.013.5602221
CACGCTC200300.013.2802449
GTCCGCT47150.013.2571751
Produced by FastQC (version 0.11.5)