FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853096

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853096
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49686346
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT750200.15098715449914552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT165600.022.83920145
ACCGCGT170050.022.3758416
AGACCGC172850.022.1695924
GACCGCG175600.021.6942625
CCGCGTT180700.021.0676337
AACGCTA125250.020.96363823
ACGCTAC127450.020.53115524
CAGACCG190600.020.30573
CGCGTTC187200.020.2880428
CGCTACC133750.019.42950425
AGAACGA199100.019.07544544
AAAACGC145100.018.26633621
AATACGG207250.018.12990232
TCAGACC223300.017.6747512
TACGGAG215800.017.4220234
ATACGGA219950.017.19559933
CAATACG225700.016.87714431
GCGTTCT229100.016.6364969
AAACGCT163500.016.30700322
GAAAACG165700.016.15838620
Produced by FastQC (version 0.11.5)