FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004853099

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004853099
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56306294
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG51600.015.35123726
GGAGGTC267100.013.0321454
GGGAGGT276900.012.9944223
ATATAGC73250.012.8086916
TGTCGTT63600.012.73795228
GCTAAGT71300.011.92672214
AGCTTAG248400.011.80328945
TCAGACG97200.011.7144922
GGCATAG247150.011.61713935
TATAGCT82100.011.5923427
GTCACTC287300.011.5510828
ATAGGGG263600.011.36918538
GAGGTCA314300.011.1604465
ACTCTCC304800.011.07116111
GGTCACT304050.011.011137
GTCGTTC74850.010.94371129
AGGTCAC321950.010.8602286
CATAGGG267200.010.69431137
TCGTTCC75900.010.6144230
GAGCTTA280150.010.4633444
Produced by FastQC (version 0.11.5)